Protection against lethal influenza with neuraminidase
Identifieur interne : 002207 ( Main/Exploration ); précédent : 002206; suivant : 002208Protection against lethal influenza with neuraminidase
Auteurs : R. G. Webster [États-Unis] ; P. A. Reay [Royaume-Uni] ; W. G. Laver [Australie]Source :
- Virology [ 0042-6822 ] ; 1988.
English descriptors
- Teeft :
- Academic press, Active center, Adjuvant, Adult birds, Antibody, Antibody titer, Antigenic, Antigenic hybrids, Antigenic sites, Antigenic variants, Birds vaccinated, Catalytic site, Chickens vaccinated, Clinical signs, Competitive binding assays, Concave enzyme, Contact birds, Determinant, Disease signs, Enzyme activity, Epitope, Generalized infection, High levels, Influenza, Influenza virus, Influenza virus neuraminidase, Influenza virus strains, Influenza viruses, Inoculated, Insufficient antibody, Jude research hospital, Laver, Lethal infection, Lethal influenza, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Neuraminidase, Neuraminidaseexpressed vaccinia, Nonimmune birds, Nonvaccinated birds, Parental virus, Partial protection, Passive transfer, Recent infection, Recombinant, Replication, Serum samples, Studies show, Trachea, Vaccinated, Vaccinated birds, Vaccine, Vaccinia, Variant, Virology, Virulent, Virulent influenza virus, Virulent influenza viruses, Virulent virus, Virus, Virus levels, Virus replication, Virus titers, Webster.
Abstract
Abstract: The role of the neuraminidase in eliciting protection against a lethal influenza A virus [A/Ck/Penn/1370/83 (H5N2)] infection was investigated in chickens. Isolated N2 neuraminidase administered in adjuvant did not prevent infection but did prevent systemic spread of virus and death of chickens. N2 expressed in a recombinant vaccinia virus protected chickens when administered in adjuvant but was less effective when allowed to replicate and produce pox on the chicken's comb. Chickens vaccinated with isolated N2 in adjuvant or with inactivated H5N2 influenza virus were protected from clinical signs and death after challenge with A/Ck/Penn/1370/83 influenza virus. However, these animals were completely susceptible and died of infection with a heterologous subtype (H7N7) of influenza virus. The role of the different antigenic determinants on the N2 NA was investigated in chickens by passive transfer of monoclonal antibodies. Antibodies to antigenic determinants rimming the enzyme active center reduced disease signs in approximately half of the birds but did not significantly reduce virus levels. Antibodies to one of the two independent antigenic determinants that are distant from the enzyme active center were most effective at reducing virus replication and disease signs. This is surprising because antigenic variants could not be selected in vitro with these antibodies and suggests that they may facilitate clearance of virus. Antibodies to the other determinant that is located distally to the enzyme site were ineffective at providing protection.
Url:
DOI: 10.1016/0042-6822(88)90640-X
Affiliations:
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Reay</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, England</wicri:regionArea><orgName type="university">Université d'Oxford</orgName><placeName><settlement type="city">Oxford</settlement><region type="nation">Angleterre</region><region type="région" nuts="1">Oxfordshire</region></placeName></affiliation></author><author><name sortKey="Laver, W G" sort="Laver, W G" uniqKey="Laver W" first="W. G." last="Laver">W. G. Laver</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Australie</country><wicri:regionArea>Influenza Virus Immunochemistry Unit, John Curtin Medical School, Australian National University, P.O. Box 334, Canberra City, A.C.T. 2601</wicri:regionArea><wicri:noRegion>A.C.T. 2601</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1988">1988</date><biblScope unit="volume">164</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="230">230</biblScope><biblScope unit="page" to="237">237</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Active center</term><term>Adjuvant</term><term>Adult birds</term><term>Antibody</term><term>Antibody titer</term><term>Antigenic</term><term>Antigenic hybrids</term><term>Antigenic sites</term><term>Antigenic variants</term><term>Birds vaccinated</term><term>Catalytic site</term><term>Chickens vaccinated</term><term>Clinical signs</term><term>Competitive binding assays</term><term>Concave enzyme</term><term>Contact birds</term><term>Determinant</term><term>Disease signs</term><term>Enzyme activity</term><term>Epitope</term><term>Generalized infection</term><term>High levels</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus neuraminidase</term><term>Influenza virus strains</term><term>Influenza viruses</term><term>Inoculated</term><term>Insufficient antibody</term><term>Jude research hospital</term><term>Laver</term><term>Lethal infection</term><term>Lethal influenza</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Monoclonal antibody</term><term>Neuraminidase</term><term>Neuraminidaseexpressed vaccinia</term><term>Nonimmune birds</term><term>Nonvaccinated birds</term><term>Parental virus</term><term>Partial protection</term><term>Passive transfer</term><term>Recent infection</term><term>Recombinant</term><term>Replication</term><term>Serum samples</term><term>Studies show</term><term>Trachea</term><term>Vaccinated</term><term>Vaccinated birds</term><term>Vaccine</term><term>Vaccinia</term><term>Variant</term><term>Virology</term><term>Virulent</term><term>Virulent influenza virus</term><term>Virulent influenza viruses</term><term>Virulent virus</term><term>Virus</term><term>Virus levels</term><term>Virus replication</term><term>Virus titers</term><term>Webster</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The role of the neuraminidase in eliciting protection against a lethal influenza A virus [A/Ck/Penn/1370/83 (H5N2)] infection was investigated in chickens. Isolated N2 neuraminidase administered in adjuvant did not prevent infection but did prevent systemic spread of virus and death of chickens. N2 expressed in a recombinant vaccinia virus protected chickens when administered in adjuvant but was less effective when allowed to replicate and produce pox on the chicken's comb. Chickens vaccinated with isolated N2 in adjuvant or with inactivated H5N2 influenza virus were protected from clinical signs and death after challenge with A/Ck/Penn/1370/83 influenza virus. However, these animals were completely susceptible and died of infection with a heterologous subtype (H7N7) of influenza virus. The role of the different antigenic determinants on the N2 NA was investigated in chickens by passive transfer of monoclonal antibodies. Antibodies to antigenic determinants rimming the enzyme active center reduced disease signs in approximately half of the birds but did not significantly reduce virus levels. Antibodies to one of the two independent antigenic determinants that are distant from the enzyme active center were most effective at reducing virus replication and disease signs. This is surprising because antigenic variants could not be selected in vitro with these antibodies and suggests that they may facilitate clearance of virus. Antibodies to the other determinant that is located distally to the enzyme site were ineffective at providing protection.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Oxfordshire</li></region><settlement><li>Oxford</li></settlement><orgName><li>Université d'Oxford</li></orgName></list><tree><country name="États-Unis"><noRegion><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R. G." last="Webster">R. G. Webster</name></noRegion></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Reay, P A" sort="Reay, P A" uniqKey="Reay P" first="P. A." last="Reay">P. A. Reay</name></region></country><country name="Australie"><noRegion><name sortKey="Laver, W G" sort="Laver, W G" uniqKey="Laver W" first="W. G." last="Laver">W. G. Laver</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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